Indeed, the clash with Gloucester marks a definitive moment for the Titans' history. Not only does it signal the start of their Premiership campaign, but it also sees the official first team debut of seven players, a club record.
Christian Loader, Luke Gross, Geraint Lewis, Jason Strange, Pete Jorgensen, Jon Pritchard and Anthony Elliot, many of whom have been brought into the club over the summer, will all make their Premiership debuts for the Titans.
Captain Chris Johnson is convinced that the team will stand up well against formidable opposition: "This Saturday is a stand out moment for Rotherham Titans and for us all, especially the seven debutants, it will undoubtedly be an unforgettable match.
"I have no doubt that we will give a good account of ourselves and hopefully mark our return to the Zurich Premiership in style."
Colin Noon, Derek Jelley and James Craig are absent through injury, and Joe Ewens, a former Gloucester player, through illness.
Rotherham Titans: 15 Jon Benson, 14 Anthony Elliot, 13 Jon Pritchard, 12 Peter Jorgensen, 11 Michael Wood, 10 Jason Strange, 9 Jacob Rauluni, 8 Geraint Lewis, 7 Neil Spence, 6 Leon Greeff, 5 Luke Gross, 4 Dan Cook, 3 Chris Loader, 2 Chris Johnson (captain), 1 Nick Lloyd. Replacements: 16 Peter Smyth, 17 Simon Bunting, 18 Glen Kenworthy, 19 James Cockle, 20 Charlie Harrison, 21 Phil Jones, 22 Matt Oliver.
Kick-off: 15.00 BST (13.00 GMT)