The Tigers move from their Welford Road home in round two to face one of their oldest rivals in Bath, a match which celebrates the 100th anniversary of games between the two sides. The first match was on 13 September 1913.
The opening weekend of the Aviva Premiership Rugby season will kick off on Friday 6 September when newly-promoted Newcastle Falcons have the honour of opening the new campaign.
The clash between Newcastle Falcons and Bath Rugby has been chosen as the first Aviva Premiership Rugby match to feature live on BT Sport, at Kingston Park, with a 7.45pm kick off.
The Saturday of the first weekend will feature the traditional London Double Header which sees London Irish kick off against Saracens (2pm), followed by London Wasps against Harlequins (4.30pm), both live on BT Sport.
To complete a stunning opening weekend, last season's Aviva Premiership Rugby runners-up Northampton Saints entertain Exeter Chiefs while Steve Diamond's Sale Sharks make the trip to Kingsholm to face Gloucester Rugby - both matches are on Saturday.
"Every year the anticipation over the new fixtures grows and looking at the 2013-14 season it is clear we have huge matches every weekend," said Phil Winstanley the Rugby Director at Premiership Rugby.
"Matches in Aviva Premiership Rugby get more compelling and competitive every year and last season we once again had a smaller average winning margin (a slim 11 points) in our games than in any other League in the world.
"We've had three different Aviva Premiership champions in the last three years and last May we had a new club (Northampton Saints) in the Aviva Premiership Rugby Final."
This season there will be two Aviva Premiership Rugby matches at Twickenham - in addition to the London Double Header and the Final - and one at Wembley. Harlequins face Exeter Chiefs on 28 December and London Wasps entertain Gloucester Rugby on 19 April at Twickenham, while Saracens will play their match with Harlequins on 22 March at Wembley. In the 2011-12 season the Saracens v Harlequins match at Wembley attracted a world record crowd for a club rugby match of 83,761 supporters.
Simon Green, Head of BT Sport, said: "BT Sport is the new home of club rugby and we'll be broadcasting 69 live and exclusive Aviva Premiership Rugby games across the season. Friday night's coverage will be anchored live from the grounds with games broadcast live on a Saturday and Sunday from the BT Sport Studio."
"We have a fantastic rugby team in place. Hosted by Craig Doyle, commentary will be provided by Nick Mullins and Alastair Eykyn who will be joined by BT Sport rugby experts, Lawrence Dallaglio, Ben Kay, Matt Dawson and Austin Healey. Reporting duties fall to Sarra Elgan and Martin Bayfield.
"I'm also delighted to announce a new Wednesday night show, Rugby Tonight, which will be hosted by Matt Dawson, Craig Doyle, Martin Bayfield and Sarra Elgan, which will offer a mixture of rugby analysis, debate and lively entertainment."
Heather Smith, UK Direct Insurance & Ireland Marketing Director at Aviva said: "We are delighted to be sponsoring our fourth season of Aviva Premiership Rugby.
"To see three different winners in the last three years has been fantastic and shows just how competitive the Aviva Premiership has become. We are happy to be extending our partnership with the sport through to 2017. Good luck to all the teams involved in the coming season."
For a full fixture list, click here.